JOVE JOURNAL

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Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Nicholas W. Chavkin^1,2, Shelby Cain¹, Kenneth Walsh^2,3,4, Karen K. Hirschi^1,2,4,5

¹Department of Cell Biology,University of Virginia School of Medicine, ²Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine, ³Department of Cardiology,University of Virginia School of Medicine, ⁴Hematovascular Biology Center,University of Virginia School of Medicine, ⁵Yale Cardiovascular Research Center,Yale University School of Medicine

Journal (Developmental Biology)

In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth

Hanna M. Alcocer¹, Xiaoxing Xu¹, Morgan E. Gravely¹, John M. Gonzalez¹

¹Department of Animal and Dairy Sciences,University of Georgia

Journal (Developmental Biology)

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Ruby Boateng¹, Nathaniel Boechat¹, Philipp P. Henrich¹, Ewelina Bolcun-Filas¹

¹The Jackson Laboratory

Journal (Developmental Biology)

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Frederic Català-Castro¹, Valeria Venturini^2,3, Santiago Ortiz-Vásquez¹, Verena Ruprecht^2,4, Michael Krieg¹

¹Neurophotonics and Mechanical Systems Biology,Institut de Ciències Fotòniques, ICFO, ²Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, ³Institut de Ciències Fotòniques, ICFO, ⁴Universitat Pompeu Fabra (UPF)

Journal (Developmental Biology)

Direct Reprogramming of Mouse Fibroblasts into Melanocytes

Yi-Xuan Zhang^1,2, Li-Ping Liu^1,2, Ming Jin^1,2, Hui Sun^1,2, Han-Lin Zhang^1,2, Yu-Mei Li^1,2

¹Institute for Regenerative Medicine,Jiangsu University, ²Department of Dermatology,Affiliated Hospital of Jiangsu University

Journal (Developmental Biology)

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Jesselynn LaBelle¹, Stephanie Woo¹

¹Department of Molecular Cell Biology,University of California

Journal (Developmental Biology)

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Sagar N. Kasar¹, Sarah A. Grandinette¹, Scott D. Semelsberger¹, Cuong Q. Diep¹

¹Department of Biology,Indiana University of Pennsylvania

Journal (Developmental Biology)

An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

Tram P. Le*¹, Xiaolei Zhao*¹, Shannon Erhardt^1,2, Jianhua Gu³, Huie Wang³, Tina O. Findley¹, Jun Wang^1,2

¹Department of Pediatrics, McGovern Medical School,The University of Texas Health Science Center at Houston, ²The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences,The University of Texas Health Science Center at Houston, ³SEM AFM Core in the Houston Methodist Hospital Research Institute

Journal (Developmental Biology)

A Simple and Effective Transplantation Device for Zebrafish Embryos

Gary H. Soh¹, Anna C. Kögler¹, Patrick Müller^1,2

¹Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society, ²University of Konstanz

Journal (Developmental Biology)

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Alyssa Alaniz Emig¹, Margot L. K. Williams¹

¹Center for Precision Environmental Health and Department of Molecular and Cellular Biology,Baylor College of Medicine

Journal (Developmental Biology)

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

Hyung-Seok Kim¹, Jan L. Christian²

¹Department of Neurobiology,University of Utah, ²Departments of Neurobiology and Department of Internal Medicine, Division of Hematology and Hematologic Malignancies,University of Utah, School of Medicine

Journal (Developmental Biology)

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Arthur Boutillon¹, Sophie Escot¹, Nicolas B. David¹

¹Laboratory for Optics and Biosciences,CNRS UMR7645, INSERM U1182, Institut Polytechnique de Paris

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