JOVE JOURNAL

생화학

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Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Satyaghosh Maurya¹, Vishwesh Haricharan Rai¹, Aditya Upasani¹, Saurabh Umrao^1,2, Diksha Parwana¹, Rahul Roy^1,3

¹Department of Chemical Engineering,Indian Institute of Science, ²Holonyak Micro & Nanotechnology Lab,University of Illinois, Urbana-Champaign, ³Center for BioSystems Science and Engineering,Indian Institute of Science

Journal (Biochemistry)

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Sriram Aiyer¹, Timothy S. Strutzenberg¹, Marianne E. Bowman², Joseph P. Noel^2,3, Dmitry Lyumkis^1,4,5

¹Laboratory of Genetics,The Salk Institute for Biological Studies, ²Jack H. Skirball Center for Chemical Biology and Proteomics,The Salk Institute for Biological Studies, ³Department of Chemistry and Biochemistry,University of California San Diego, ⁴Graduate School of Biological Sciences, Section of Molecular Biology,University of California San Diego, ⁵Department of Integrative Structural and Computational Biology,The Scripps Research Institute

Journal (Biochemistry)

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

Matthew C. Johnson*^1,2, Arshay J. Grant*¹, Ingeborg Schmidt-Krey^1,3

¹School of Biological Sciences,Georgia Institute of Technology, ²Department of Structural Biology,Genentech, ³School of Chemistry and Biochemistry,Georgia Institute of Technology

Journal (Biochemistry)

Self-Assembly of Microtubule Tactoids

Prashali Chauhan¹, Sumon Sahu^1,2, Niaz Goodbee¹, Sophia Martin¹, Hong Beom Lee¹, Ruell Branch¹, Jennifer M. Schwarz¹, Jennifer L. Ross¹

¹Physics Department,Syracuse University, ²Department of Molecular Biophysics and Biochemistry,Yale School of Medicine

Journal (Biochemistry)

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Gerard Castro-Linares¹, Jeffrey den Haan¹, Francois Iv², Carla Silva Martins², Aurélie Bertin³, Manos Mavrakis², Gijsje H. Koenderink¹

¹Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology, ²Institut Fresnel, CNRS UMR7249,Aix Marseille Univ, Centrale Marseille, ³Institut Curie, Université PSL,Sorbonne Université

Journal (Biochemistry)

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Lin Cheng*¹, Lantian Su*¹, Xiaowen Tian*¹, Fan Xia², Chang Zhao¹, Wei Yan¹, Zhenhua Shao¹

¹Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital,Sichuan University, ²Department of Neurosurgery, West China Hospital,Sichuan University

Journal (Biochemistry)

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Elizabeth M. Bailey¹, Emanuel Salazar-Cavazos¹, Rachel M. Grattan¹, Michael J. Wester², David J. Schodt², Julian A. Rojo¹, Keith A. Lidke^2,3, Diane S. Lidke^1,3

¹Department of Pathology,University of New Mexico School of Medicine, ²Department of Physics and Astronomy,University of New Mexico, ³Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Journal (Biochemistry)

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Armand M. G. Jaminon*¹, Nikolas Rapp*¹, Asim C. Akbulut¹, Robert Dzhanaev², Chris P. Reutelingsperger¹, Willi Jahnen-Dechent², Leon J. Schurgers^1,3

¹Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, ²Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, ³Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Journal (Biochemistry)

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Joann Phan¹, Joseph Kapcia III¹, Cynthia I. Rodriguez², Victoria L. Vogel³, Daniel B Cardin³, Sage J. B. Dunham³, Katrine Whiteson¹

¹Department of Molecular Biology and Biochemistry,University of California Irvine, ²Department of Ecology and Evolutionary Biology,University of California Irvine, ³Entech Instruments Inc.

Journal (Biochemistry)

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass

Kenneth J. Olejar*¹, Min Hong*², Sun-Yeop Lee², Tae-Hyung Kwon², Soo-Ung Lee², Chad A. Kinney^1,3, Joon-Hee Han², Sang-Hyuck Park³

¹Department of Chemistry,Colorado State University-Pueblo, ²Chuncheon Bioindustry Foundation, ³Institute for Cannabis Research,Colorado State University-Pueblo

Journal (Biochemistry)

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Khanit Sa-ngiamsuntorn¹, Piyanoot Thongsri², Yongyut Pewkliang², Suparerk Borwornpinyo^3,4, Adisak Wongkajornsilp⁵

¹Department of Biochemistry, Faculty of Pharmacy,Mahidol University, ²Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, ³Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, ⁴Department of Biotechnology, Faculty of Science,Mahidol University, ⁵Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University

Journal (Biochemistry)

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Patrícia M. S. dos Passos*¹, Valentine Spagnol*², Camila R.S.T.B de Correia¹, Felipe R. Teixeira^1,2

¹Department of Genetic and Evolution,Federal University of São Carlos, ²Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto,University of São Paulo

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