Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Marie Bannier-H&#233;laou&#235;t; Maarten H. Geurts; Jeroen Korving; Harry Begthel; Hans Clevers

doi:10.3791/65040

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Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

JoVE Journal
生物学

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JoVE Journal 生物学

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

2,238 Views

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10:49 min

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February 03, 2023

DOI:

10.3791/65040-v

DOI:

10.3791/65040-v

•

10:49 min

February 03, 2023

•

37 Views

Marie Bannier-Hélaouët^1,2, Maarten H. Geurts^1,2, Jeroen Korving¹, Harry Begthel¹, Hans Clevers^1,2

¹Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),University Medical Center Utrecht, ²Oncode Institute,Hubrecht Institute

概要

This protocol describes how to establish, maintain, genetically modify, differentiate, functionally characterize, and transplant lacrimal gland organoids derived from primary mouse and human tissue.

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Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

概要

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