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Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
JoVE Journal
Biologie
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JoVE Journal Biologie
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

2,238 Views

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10:49 min

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February 03, 2023

DOI:

10.3791/65040-v

DOI:
10.3791/65040-v

•

10:49 min
February 03, 2023

•

37 Views
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1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),University Medical Center Utrecht, 2Oncode Institute,Hubrecht Institute

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This protocol describes how to establish, maintain, genetically modify, differentiate, functionally characterize, and transplant lacrimal gland organoids derived from primary mouse and human tissue.

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Bannier-Hélaouët, M., Geurts, M. H., Korving, J., Begthel, H., Clevers, H. Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids. J. Vis. Exp. (192), e65040, doi:10.3791/65040 (2023).

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