The secretion of root exudates is usually an external detoxification strategy for plants under stress conditions. This protocol describes how to assess the impact of xenobiotics on alfalfa via nontargeted metabolomic analysis.
Ren, W., Zhao, R., Teng, Y., Luo, Y. Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production. J. Vis. Exp. (196), e64470, doi:10.3791/64470 (2023).