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Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production
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Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production

Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production

1,325 Views

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06:46 min

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June 02, 2023

DOI:

10.3791/64470-v

DOI:
10.3791/64470-v

•

06:46 min
June 02, 2023

•

14 Views
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1Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science,Chinese Academy of Sciences, 2College of Resources and Environment,University of Chinese Academy of Sciences

Summary

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The secretion of root exudates is usually an external detoxification strategy for plants under stress conditions. This protocol describes how to assess the impact of xenobiotics on alfalfa via nontargeted metabolomic analysis.

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AlfalfaRoot ExudatesDi(2-ethylhexyl) PhthalateDEHPXenobioticsMetabolomicsMedicago SativaSeed SterilizationGerminationRoot Exudate CollectionMetabolite ProfilingExtractionCentrifugation

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Cite this Article

Ren, W., Zhao, R., Teng, Y., Luo, Y. Collection of Alfalfa Root Exudates to Study the Impact of Di(2-ethylhexyl) Phthalate on Metabolite Production. J. Vis. Exp. (196), e64470, doi:10.3791/64470 (2023).

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