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Microinjeções ventriculares cerebrais de lipopolissacarídeo em peixe-zebra larval para avaliar a neuroinflamação e a neurotoxicidade
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JoVE Journal 神経科学
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Microinjeções ventriculares cerebrais de lipopolissacarídeo em peixe-zebra larval para avaliar a neuroinflamação e a neurotoxicidade

2,670 Views

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07:31 min

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August 23, 2022

DOI:

10.3791/64313-v

DOI:
10.3791/64313-v

•

07:31 min
August 23, 2022

•

18 Views
,
1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences,University of Macau, Avenida da Universidade, 2Department of Pharmaceutical Sciences, Faculty of Health Sciences,University of Macau, Avenida da Universidade

概要

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Este protocolo demonstra a microinjeção de lipopolissacarídeo na região ventricular cerebral em um modelo larval de peixe-zebra para estudar a resposta neuroinflamatória e a neurotoxicidade resultantes.

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Brain VentricleMicroinjectionLipopolysaccharideNeuroinflammationNeurotoxicityZebrafishIn Vivo ModelAnti-neuroinflammatory DrugsGlass CapillaryMicromanipulatorAgaroseAnesthesia

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Cite this Article

He, Y., Lee, S. M. Y. Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity. J. Vis. Exp. (186), e64313, doi:10.3791/64313 (2022).

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