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Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography
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Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography

Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography

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09:41 min

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January 20, 2023

DOI:

10.3791/64133-v

DOI:
10.3791/64133-v

•

09:41 min
January 20, 2023

•

1 Views
, , , , , ,
1Department of Radiology,Stanford University, 2Department of Neurology and Neurological Sciences,Stanford University

概要

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This paper details methodology for radiolabeling a human-specific anti-CD19 monoclonal antibody and how to use it to quantify B cells in the central nervous system and peripheral tissues of a mouse model of multiple sclerosis using in vivo PET imaging, ex vivo gamma counting, and autoradiography approaches.

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Cite this Article

Reyes, S. T., Azevedo, E. C., Cropper, H. C., Nagy, S., Deal, E. M., Chaney, A. M., James, M. L. Imaging CD19+ B Cells in an Experimental Autoimmune Encephalomyelitis Mouse Model using Positron Emission Tomography. J. Vis. Exp. (191), e64133, doi:10.3791/64133 (2023).

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