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制备与DNA修复因子络合的核小体核心颗粒用于冷冻电镜结构测定
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生化学
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JoVE Journal 生化学
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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制备与DNA修复因子络合的核小体核心颗粒用于冷冻电镜结构测定

1,509 Views

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07:59 min

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August 17, 2022

DOI:

10.3791/64061-v

DOI:
10.3791/64061-v

•

07:59 min
August 17, 2022

•

5 Views
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1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

概要

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该协议详细概述了使用两种用于冷冻TEM网格的样品制备方法制备核小体复合物。

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NucleosomeDNA RepairCryo-electron MicroscopySample PreparationCross-linkingSize Exclusion ChromatographyBuffer ExchangeProtein Complex

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Cite this Article

Rodriguez, Y., Butay, K. J., Sharma, K., Viverette, E., Wilson, S. H. Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination. J. Vis. Exp. (186), e64061, doi:10.3791/64061 (2022).

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