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Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis
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JoVE Journal 工学
Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

2,086 Views

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08:33 min

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August 11, 2022

DOI:

10.3791/63850-v

DOI:
10.3791/63850-v

•

08:33 min
August 11, 2022

•

5 Views
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1Department of Biomedical Engineering,Foundation University Islamabad, 2College of Medical Technology,The Islamic University Najaf, 3Faculty of Mechanical Engineering,University of Tabriz, 4Department of Engineering Mathematics,University of Bristol, 5School of Life Sciences,University of Warwick

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Breast cancer cells exhibit different dielectric properties compared to non-tumor breast epithelial cells. It has been hypothesized that, based on this difference in dielectric properties, the two populations can be separated for immunotherapy purposes. To support this, we model a microfluidic device to sort MCF-7 and MCF-10A cells.

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Microfluidic DeviceAC DielectrophoresisNon-metastatic Breast Cancer CellsNon-tumor Breast Epithelial CellsCell SeparationDielectric PropertiesMultiphysics SimulationParticle TracingFluid FlowElectric Field

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Cite this Article

ur Rehman, A., Zabibah, R. S., Kharratian, S., Mustafa, A. Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis. J. Vis. Exp. (186), e63850, doi:10.3791/63850 (2022).

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