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Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
章
- 00:04Introduction
- 00:54Preparation of In Vitro Biofilm
- 02:34Measurement of ROS Production by Neutrophils
- 04:14Imaging the Biofilm-Neutrophil Interactions
- 05:31Results: Assessing Neutrophils and Biofilms Interactions via Standardized In Vitro Assays
- 06:56Conclusion
The present protocol describes the study of neutrophil-biofilm interactions. Staphylococcus aureus biofilms are established in vitro and incubated with peripheral blood-derived human neutrophils. The oxidative burst response from neutrophils is quantified, and the neutrophil localization within the biofilm is determined by microscopy.
