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Immunology and Infection

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JoVE Journal Immunology and Infection
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
 

Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

Article DOI: 10.3791/63773-v • 07:28 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1,2
1Department of Microbiology, The Ohio State University, 2Department of Microbial Infection and Immunity, The Ohio State University

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The present protocol describes the study of neutrophil-biofilm interactions. Staphylococcus aureus biofilms are established in vitro and incubated with peripheral blood-derived human neutrophils. The oxidative burst response from neutrophils is quantified, and the neutrophil localization within the biofilm is determined by microscopy.

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In Vitro Assays Human Neutrophils Staphylococcus Aureus Biofilms Interactions Visualize Quantify Standardized Protocols Assay Variability Donor Variation Biofilm Formation Streak Plate Technique Nutrient-rich Agar Plate PLL Coating Sterile Water Overnight Culture MEM-alpha Medium Glucose Supplementation Incubation Conditions
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