ジャーナル
/
神経科学
/
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
JoVE Journal
神経科学
This content is Free Access.
JoVE Journal 神経科学
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

DOI:
10.3791/63574-v

•

05:35 min

•

May 31, 2022

•

, , ,
1Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 2GLIA Center,University of Yamanashi

章

  • 00:04Introduciton
  • 01:04Preparation of iPSMG
  • 02:01Transnasal Transplantation
  • 03:43Results: Viability of iPSMG in the Cortex and the Hippocampus
  • 04:27Conclusion

概要

自動翻訳

自動翻訳

The protocol presented here allows the transplantation of induced pluripotent stem cell-derived human microglia (iPSMG) into the brain via a transnasal route in immunocompetent mice. The method for the preparation and transnasal transplantation of cells and the administration of cytokine mixture for the maintenance of iPSMG is shown.

タグ

Induced Pluripotent Stem Cell-derived MicrogliaTransnasal TransplantationImmunocompetent MiceCSF1 Receptor Antagonist PLX5622Non-invasiveNeurodegenerative DiseasesHyaluronidaseCell Transplantation

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Low-stress Route Learning Using the Lashley III Maze in Mice

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Obtaining Human Microglia from Adult Human Brain Tissue

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

Read Article