このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
3,041 Views
•
09:39 min
•
March 31, 2022
DOI:
概要
Automatically generated
Field-effect biosensing (FEB) is a label-free technique for detecting biomolecular interactions. It measures the electric current through the graphene biosensor to which the binding targets are immobilized. The FEB technology was used to evaluate biomolecular interactions between Hsp90 and Cdc37 and a strong interaction between the two proteins was detected.
Explore More Videos
Biomolecular InteractionMolecular Chaperone Hsp90Client Protein Kinase Cdc37Field-effect Biosensing TechnologyFEBLabel-freeCost-effectiveInteraction DetectionInhibitor TestingAutomatedUser-friendlyPharmaceuticalsBiomolecular AnalysisDrug ValidationChip FunctionalizationEDCSulfo-NHSMES BufferPBSTarget MoleculeQuench Solution
Read Article
Cite this Article
Lerner, Y., Sukumaran, S., Chua, M., So, S. K., Qvit, N. Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology. J. Vis. Exp. (181), e63495, doi:10.3791/63495 (2022).
Copy