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Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
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Biochemie
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JoVE Journal Biochemie
Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

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09:39 min

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March 31, 2022

DOI:

10.3791/63495-v

DOI:
10.3791/63495-v

•

09:39 min
March 31, 2022

•

8 Views
, , , ,
1The Azrieli Faculty of Medicine in the Galilee,Bar-Ilan University, 2Asian Liver Center, Department of Surgery,Stanford University School of Medicine

Summary

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Field-effect biosensing (FEB) is a label-free technique for detecting biomolecular interactions. It measures the electric current through the graphene biosensor to which the binding targets are immobilized. The FEB technology was used to evaluate biomolecular interactions between Hsp90 and Cdc37 and a strong interaction between the two proteins was detected.

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Lerner, Y., Sukumaran, S., Chua, M., So, S. K., Qvit, N. Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology. J. Vis. Exp. (181), e63495, doi:10.3791/63495 (2022).

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