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Differenziazione di cellule staminali pluripotenti umane in precursori di cellule beta pancreatiche in un sistema di coltura 2D
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発生生物学
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JoVE Journal 発生生物学
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
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Differenziazione di cellule staminali pluripotenti umane in precursori di cellule beta pancreatiche in un sistema di coltura 2D

2,434 Views

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10:12 min

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December 16, 2021

DOI:

10.3791/63298-v

DOI:
10.3791/63298-v

•

10:12 min
December 16, 2021

•

14 Views
,
1College of Health and Life Sciences,Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI),Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

概要

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Il presente protocollo descrive un metodo potenziato per aumentare la co-espressione dei fattori di trascrizione PDX1 e NKX6.1 nei progenitori pancreatici derivati da cellule staminali pluripotenti umane (hPSC) in monostrati planari. Ciò si ottiene reintegrando la matrice fresca, manipolando la densità cellulare e dissociando le cellule endodermiche.

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Cite this Article

Memon, B., Abdelalim, E. M. Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System. J. Vis. Exp. (178), e63298, doi:10.3791/63298 (2021).

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