ジャーナル
/
神経科学
/
Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

DOI:
10.3791/63283-v

•

04:06 min

•

November 30, 2021

•

, ,
1Department of Chemistry, Faculty of Science,Hong Kong Baptist University

章

  • 00:05Introduction
  • 00:42Day In Vitro (DIV) 0: Electroporation for Overexpression of Hedgehog Receptor: pEGFP-Smo
  • 02:28Results: Assessment of Overexpression Efficiency and the Effects of SAG Treatment on Primary Cilium-Dependent Hedgehog Signaling Pathway
  • 03:23Conclusion

概要

自動翻訳

自動翻訳

Here, we present a reproducible in vitro electroporation protocol for genetic manipulation of primary cerebellar granule cell precursors (GCPs) that is cost-effective, efficient, and viable. Moreover, this protocol also demonstrates a straightforward method for the molecular study of primary cilium-dependent Hedgehog signaling pathways in primary GCP cells.

タグ

ElectroporationPrimary CiliumGranule Cell PrecursorArl13bPax6In Vitro Genetic ModificationCost-effectiveEfficientSignaling Pathway

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Design and Construction of a Cost Effective Headstage for Simultaneous Neural Stimulation and Recording in the Water Maze

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

Read Article