Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Efficient and Cost Effective Electroporation…
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor
 

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

Article DOI: 10.3791/63283-v • 04:06 min • November 30th, 2021
November 30th, 2021 •
1, 1, 1
1Department of Chemistry, Faculty of Science, Hong Kong Baptist University

Kapitel

Summary
Automatic Translation

Here, we present a reproducible in vitro electroporation protocol for genetic manipulation of primary cerebellar granule cell precursors (GCPs) that is cost-effective, efficient, and viable. Moreover, this protocol also demonstrates a straightforward method for the molecular study of primary cilium-dependent Hedgehog signaling pathways in primary GCP cells.

Tags

Efficient Cost-effective Electroporation Method Primary Cilium-dependent Signaling Pathways Granule Cell Precursor Genetic Modification Method Transfection Method Electroporation Technique Primary GCP Cultures Culture Medium Carbon Dioxide Incubator Microcentrifuge Tube Opti-MEM Electroporation Parameters Electroporation Reaction Cuvette Chamber Electroporator
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter