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Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
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JoVE Journal 免疫学と感染
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

2,396 Views

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09:42 min

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October 07, 2021

DOI:

10.3791/63023-v

DOI:
10.3791/63023-v

•

09:42 min
October 07, 2021

•

5 Views
,
1Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles

概要

Automatically generated

The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.

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Cite this Article

Castelletto, M. L., Hallem, E. A. Generating Transgenics and Knockouts in Strongyloides Species by Microinjection. J. Vis. Exp. (176), e63023, doi:10.3791/63023 (2021).

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