Rivista
/
Immunologia e infezioni
/
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

2,396 Views

•

09:42 min

•

October 07, 2021

DOI:

10.3791/63023-v

DOI:
10.3791/63023-v

•

09:42 min
October 07, 2021

•

5 Views
,
1Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles

Summary

Automatically generated

The functional genomic toolkit for the parasitic nematodes Strongyloides stercoralis and Strongyloides ratti includes transgenesis, CRISPR/Cas9-mediated mutagenesis, and RNAi. This protocol will demonstrate how to use intragonadal microinjection to introduce transgenes and CRISPR components into S. stercoralis and S. ratti.

Explore More Videos

MicroinjectionTransgenicsKnockoutsStrongyloidesParasitesWormsBaermannFecal-charcoalNematode Growth MediumE. Coli HB101

Read Article

Cite this Article

Castelletto, M. L., Hallem, E. A. Generating Transgenics and Knockouts in Strongyloides Species by Microinjection. J. Vis. Exp. (176), e63023, doi:10.3791/63023 (2021).

Download .ris file

Copy

Share Video

.

Copy