ジャーナル
/
生化学
/
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

DOI:
10.3791/62974-v

•

07:05 min

•

September 08, 2021

•

, ,
1Center for Quantitative Biology, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies,Peking University

章

  • 00:04Introduction
  • 00:58Preparation of a DNA Curtain Flowcell
  • 03:13Imaging of EWS-FLI1 Condensation Formation on DNA Curtains
  • 05:12Results: EWS-FLI1 Condensate Formation on Lambda DNA and Binding Events of the Detached Domain of mCherry-EWS-FLI1 on 25 GGAA Domain Repeats
  • 06:19Conclusion

概要

自動翻訳

自動翻訳

Here, we describe the use of the single-molecule imaging method, DNA Curtains, to study the biophysical mechanism of EWS-FLI1 condensates assembling on DNA.

タグ

Single-molecule ImagingEWS-FLI1 CondensatesDNATranscription FactorsPhase SeparationEwing SarcomaDNA MotifsP53MED1DNA CurtainLiposomeStreptavidinBiotinLambda DNAImaging BufferMicrofluidic System

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Read Article