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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
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Journal JoVE Biochimie
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

DOI:
10.3791/62974-v

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07:05 min

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September 08, 2021

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, ,
1Center for Quantitative Biology, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies,Peking University

Chapitres

  • 00:04Introduction
  • 00:58Preparation of a DNA Curtain Flowcell
  • 03:13Imaging of EWS-FLI1 Condensation Formation on DNA Curtains
  • 05:12Results: EWS-FLI1 Condensate Formation on Lambda DNA and Binding Events of the Detached Domain of mCherry-EWS-FLI1 on 25 GGAA Domain Repeats
  • 06:19Conclusion

Summary

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Here, we describe the use of the single-molecule imaging method, DNA Curtains, to study the biophysical mechanism of EWS-FLI1 condensates assembling on DNA.

Tags

Single-molecule ImagingEWS-FLI1 CondensatesDNATranscription FactorsPhase SeparationEwing SarcomaDNA MotifsP53MED1DNA CurtainLiposomeStreptavidinBiotinLambda DNAImaging BufferMicrofluidic System

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