Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Katherina Hemmen; Susobhan Choudhury; Mike Friedrich; Johannes Balkenhol; Felix Knote; Martin J. Lohse; Katrin G. Heinze

doi:10.3791/62954

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

JoVE Journal
生化学
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JoVE Journal 生化学

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

4,529 Views

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14:12 min

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December 11, 2021

DOI:

10.3791/62954-v

DOI:

10.3791/62954-v

•

14:12 min

December 11, 2021

•

20 Views

Katherina Hemmen*¹, Susobhan Choudhury*¹, Mike Friedrich¹, Johannes Balkenhol¹, Felix Knote¹, Martin J. Lohse², Katrin G. Heinze¹

¹Rudolf Virchow Center for Integrative and Translation Bioimaging,Julius-Maximilian University Wuerzburg, ²Max Delbrück Center for Molecular Medicine, Berlin

概要

We present an experimental protocol and data analysis workflow to perform live cell dual-color fluorescence cross correlation spectroscopy (FCCS) combined with Förster Resonance Energy transfer (FRET) to study membrane receptor dynamics in live cells using modern fluorescence labeling techniques.

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

概要

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