00:51Setting Up and Initiating the Stopped-Flow System
02:05Loading the Sample and Priming the System
03:01Defining the Stopped-Flow Mixing Protocol in the Program Script
03:43Defining Small-Angle Neutron Scattering (SANS) Parameters in the Instrument Control GUI
04:05Collecting Standard Scattering Measurements for Data Reduction Before Beginning the Stopped-Flow Experiment to Process the Time Resolved-SANS Data
04:47Beginning the Stopped-Flow Experiment
05:29Removing All Backgrounds, Correcting for Detector Sensitivity, Correcting for Sample Transmission,and Analyzing the Time Resolved-SANS Data
06:08Results: Raw Neutron Data and Scattered Intensities of Catalyzed Exchange of Outer Layer of Lipid Vesicles at Various Temperatures
Este protocolo apresenta o uso de um ambiente de amostra de fluxo interrompido para misturar rapidamente múltiplas soluções líquidas in situ durante uma medição de espalhamento de nêutrons de pequeno ângulo e para estudar processos cinéticos em escalas de comprimento nanométrico e segundas escalas de tempo.