00:51Setting Up and Initiating the Stopped-Flow System
02:05Loading the Sample and Priming the System
03:01Defining the Stopped-Flow Mixing Protocol in the Program Script
03:43Defining Small-Angle Neutron Scattering (SANS) Parameters in the Instrument Control GUI
04:05Collecting Standard Scattering Measurements for Data Reduction Before Beginning the Stopped-Flow Experiment to Process the Time Resolved-SANS Data
04:47Beginning the Stopped-Flow Experiment
05:29Removing All Backgrounds, Correcting for Detector Sensitivity, Correcting for Sample Transmission,and Analyzing the Time Resolved-SANS Data
06:08Results: Raw Neutron Data and Scattered Intensities of Catalyzed Exchange of Outer Layer of Lipid Vesicles at Various Temperatures
This protocol presents the use of a stopped-flow sample environment to quickly mix multiple liquid solutions in situ during a small-angle neutron scattering measurement and to study kinetic processes on nanometer length scales and second time scales.