Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Jaime Lorenzo N. Dinglasan; David T. Reeves; Robert L. Hettich; Mitchel J. Doktycz

doi:10.3791/62852

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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

JoVE Journal
生物工学

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JoVE Journal 生物工学

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

4,083 Views

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14:42 min

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September 23, 2021

DOI:

10.3791/62852-v

DOI:

10.3791/62852-v

•

14:42 min

September 23, 2021

•

29 Views

Jaime Lorenzo N. Dinglasan^1,3, David T. Reeves^2,3, Robert L. Hettich³, Mitchel J. Doktycz³

¹Graduate School of Genome Science & Technology,University of Tennessee Knoxville, ²Bredesen Center for Interdisciplinary Research,University of Tennessee Knoxville, ³Biosciences Division,Oak Ridge National Laboratory

概要

The protocols describe high-performance liquid chromatography methods coupled to refractive index or mass spectrometric detection for studying metabolic reactions in complex lysate-based cell-free systems.

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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

概要

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