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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

4,093 Views

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14:42 min

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September 23, 2021

DOI:

10.3791/62852-v

DOI:
10.3791/62852-v

•

14:42 min
September 23, 2021

•

29 Views
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1Graduate School of Genome Science & Technology,University of Tennessee Knoxville, 2Bredesen Center for Interdisciplinary Research,University of Tennessee Knoxville, 3Biosciences Division,Oak Ridge National Laboratory

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The protocols describe high-performance liquid chromatography methods coupled to refractive index or mass spectrometric detection for studying metabolic reactions in complex lysate-based cell-free systems.

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Liquid ChromatographyRefractive Index DetectionMass SpectrometryMetabolite ProfilingCell-free SystemsCarbon MetabolismCentral MetabolitesLysate-basedHPLCSample PreparationAcidificationFiltration

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Dinglasan, J. L. N., Reeves, D. T., Hettich, R. L., Doktycz, M. J. Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems. J. Vis. Exp. (175), e62852, doi:10.3791/62852 (2021).

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