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生物发光光遗传学2.0：利用生物发光在体外和体内激活光敏蛋白
JoVE Journal
生化学
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JoVE Journal 生化学
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
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生物发光光遗传学2.0：利用生物发光在体外和体内激活光敏蛋白

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07:19 min

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August 04, 2021

DOI:

10.3791/62850-v

DOI:
10.3791/62850-v

•

07:19 min
August 04, 2021

•

33 Views
, , ,
1College of Medicine,Central Michigan University, 2Biochemistry, Cell and Molecular Biology Program,Central Michigan University, 3Neuroscience Program,Central Michigan University

概要

Automatically generated

由氧化小分子底物的荧光素酶发出的生物发光 - 荧光素 - 可以用来激活光敏蛋白，从而为光刺激增加另一个维度，并能够在时间和空间尺度上操纵细胞中多种光介导的功能。

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Cite this Article

Crespo, E. L., Bjorefeldt, A., Prakash, M., Hochgeschwender, U. Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo. J. Vis. Exp. (174), e62850, doi:10.3791/62850 (2021).

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