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Tek Parçacıklı Kriyojenik Elektron Mikroskopisi için Biyolojik Örneklerin Manuel Blot ve Dalma Dondurması
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JoVE Journal 生化学
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Tek Parçacıklı Kriyojenik Elektron Mikroskopisi için Biyolojik Örneklerin Manuel Blot ve Dalma Dondurması

5,479 Views

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09:16 min

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February 07, 2022

DOI:

10.3791/62765-v

DOI:
10.3791/62765-v

•

09:16 min
February 07, 2022

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4 Views
, , ,
1Department of Chemistry and Biochemistry,University of California, San Diego

概要

Automatically generated

Bu makale, tek parçacıklı kriyojenik elektron mikroskopisi için biyolojik örnekleri manuel olarak dondurmak için leke ve dalma yöntemini özetlemektedir.

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Cite this Article

Nguyen, H. P. M., McGuire, K. L., Cook, B. D., Herzik, Jr., M. A. Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy. J. Vis. Exp. (180), e62765, doi:10.3791/62765 (2022).

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