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分离子宫先天淋巴细胞，用于流式细胞术分析
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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分离子宫先天淋巴细胞，用于流式细胞术分析

4,003 Views

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09:02 min

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October 14, 2021

DOI:

10.3791/62670-v

DOI:
10.3791/62670-v

•

09:02 min
October 14, 2021

•

8 Views
, , , , ,
1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre,University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research,University of Cambridge

概要

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这是一种从怀孕和非怀孕小鼠中分离子宫淋巴细胞的方法。该方法可用于多种下游应用，如FACS表型分析，细胞分选，功能测定，RNA-seq和蛋白质组学。这里的方案演示了如何通过流式细胞术对1组子宫先天淋巴细胞进行表型分析。

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Cite this Article

Depierreux, D. M., Seshadri, E., Shmeleva, E. V., Kieckbusch, J., Hawkes, D. A., Colucci, F. Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry. J. Vis. Exp. (176), e62670, doi:10.3791/62670 (2021).

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