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Productie van menselijke CRISPR-engineered CAR-T-cellen

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免疫学と感染

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JoVE Journal 免疫学と感染

Production of Human CRISPR-Engineered CAR-T Cells

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Productie van menselijke CRISPR-engineered CAR-T-cellen

11,756 Views

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06:33 min

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March 15, 2021

DOI:

10.3791/62299-v

DOI:

10.3791/62299-v

•

06:33 min

March 15, 2021

•

33 Views

Sangya Agarwal*^1,2, Nils Wellhausen*^1,2, Bruce L. Levine^1,2, Carl H. June^1,2

¹Center for Cellular Immunotherapies, Perelman School of Medicine,University of Pennsylvania, ²Parker Institute for Cancer Immunotherapy,University of Pennsylvania

概要

Hier presenteren we een protocol voor genbewerking in primaire menselijke T-cellen met behulp van CRISPR Cas-technologie om CAR-T-cellen te wijzigen.

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