The protocol presented here describes the use of Spotiton, a novel robotic system, to deliver two samples of interest onto a self-wicking, nanowire grid that mix for a minimum of 90 ms prior to vitrification in liquid cryogen.
Budell, W. C., Allegri, L., Dandey, V., Potter, C. S., Carragher, B. Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton. J. Vis. Exp. (168), e62271, doi:10.3791/62271 (2021).