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Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
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Biología
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JoVE Revista Biología
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

3,351 Views

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08:59 min

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February 25, 2021

DOI:

10.3791/62271-v

DOI:
10.3791/62271-v

•

08:59 min
February 25, 2021

•

4 Views
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1The National Resource for Automated Molecular Microscopy,Simons Electron Microscopy Center, New York Structural Biology Center, 2piTree Software

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The protocol presented here describes the use of Spotiton, a novel robotic system, to deliver two samples of interest onto a self-wicking, nanowire grid that mix for a minimum of 90 ms prior to vitrification in liquid cryogen.

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Cryo-electron MicroscopySpotitonRobotic SystemTime-resolved StudiesProtein ConformationMembrane Channel ActivationDNA/RNA SynthesisDrug/antibody InteractionGrid PreparationNitrogen SupplyWater ReservoirRobot InitializationTip PrimingDroplet InspectionGrid LoadingPlunge Path Camera

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Budell, W. C., Allegri, L., Dandey, V., Potter, C. S., Carragher, B. Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton. J. Vis. Exp. (168), e62271, doi:10.3791/62271 (2021).

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