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A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

DOI:
10.3791/61961-v

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06:02 min

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November 06, 2020

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, , ,
1Center for Oral and Craniofacial Biology,Louisiana State University Health-School of Dentistry, 2Department of Microbiology and Immunology,Tulane University School of Medicine

章

  • 00:04Introduction
  • 00:47Setting Up of the Warming and Restraint Device
  • 01:33Tail Vein Injection
  • 04:03Results: Sepsis Mortality vs. Vaccine-Mediated Protection Following a Lethal Challenge with Candida albicans
  • 05:23Conclusion

概要

自動翻訳

自動翻訳

Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.

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Warming DeviceRestraining DeviceTail Vein InjectionMurineFungal Sepsis ModelIntravenous InjectionVasodilationHeat TreatmentAnimal HandlingProtocolVisual Demonstration

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