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Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
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Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

2,305 Views

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08:30 min

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February 09, 2021

DOI:

10.3791/61930-v

DOI:
10.3791/61930-v

•

08:30 min
February 09, 2021

•

2 Views
, , , , ,
1生物学科,University of Florida, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3放射線腫瘍学科,Columbia University Medical Center, 4Center for Smell and Taste,University of Florida

概要

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Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

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CRISPRMutagenesisHarpegnathos SaltatorAntGenetic ModificationEmbryo InjectionMicroinjectionPlaster Nest BoxEusocial Insects行動学CommunicationPhenotype PlasticityGraduate StudentAnt RearingColony MaintenanceWaste DisposalGlass Microinjection NeedlesCasein ProteinsSmall Guide RNAs

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Cite this Article

Sieber, K., Saar, M., Opachaloemphan, C., Gallitto, M., Yang, H., Yan, H. Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator . J. Vis. Exp. (168), e61930, doi:10.3791/61930 (2021).

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