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Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
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JoVE Journal Genetik
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

2,305 Views

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08:30 min

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February 09, 2021

DOI:

10.3791/61930-v

DOI:
10.3791/61930-v

•

08:30 min
February 09, 2021

•

2 Views
, , , , ,
1Department of Biology,University of Florida, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3Department of Radiation Oncology,Columbia University Medical Center, 4Center for Smell and Taste,University of Florida

Summary

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Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

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CRISPRMutagenesisHarpegnathos SaltatorAntGenetic ModificationEmbryo InjectionMicroinjectionPlaster Nest BoxEusocial InsectsVerhaltenCommunicationPhenotype PlasticityGraduate StudentAnt RearingColony MaintenanceWaste DisposalGlass Microinjection NeedlesCasein ProteinsSmall Guide RNAs

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Sieber, K., Saar, M., Opachaloemphan, C., Gallitto, M., Yang, H., Yan, H. Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator . J. Vis. Exp. (168), e61930, doi:10.3791/61930 (2021).

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