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无激光羟基基蛋白足迹，对蛋白质进行更高级的结构分析
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JoVE Journal 生化学
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
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无激光羟基基蛋白足迹，对蛋白质进行更高级的结构分析

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09:59 min

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June 04, 2021

DOI:

10.3791/61861-v

DOI:
10.3791/61861-v

•

09:59 min
June 04, 2021

•

2 Views
, , ,
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy,University of Mississippi, 3Department of Chemistry and Biochemistry,University of Mississippi

概要

Automatically generated

该协议提出了一种使用内联基量程和等离子光源进行闪光氧化蛋白足迹的方法。该方法取代了危险的紫外激光器，简化和提高了蛋白质研究快速光化学氧化的可重复性。

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Cite this Article

Weinberger, S. R., Chea, E. E., Sharp, J. S., Misra, S. K. Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins. J. Vis. Exp. (172), e61861, doi:10.3791/61861 (2021).

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