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来自母鸡蛋的外层和内围线子层的机械分离和蛋白质溶解

JoVE Journal
生化学

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JoVE Journal 生化学

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen’s Eggs

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来自母鸡蛋的外层和内围线子层的机械分离和蛋白质溶解

4,041 Views

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06:12 min

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January 27, 2021

DOI:

10.3791/61739-v

DOI:

10.3791/61739-v

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06:12 min

January 27, 2021

•

7 Views

Mégane Bregeon¹, Nicolas Guyot¹, Sophie Réhault-Godbert¹

¹Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement,Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

概要

本文报告了一种简单的方法，用于分离鸡蛋的外层和内围线子层，同时最大限度地减少结构变化，并优化每个亚层的蛋白质溶解，以便进行蛋白质分析。

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