Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Katarina Cirnski; Janetta Coetzee; Jennifer Herrmann; Rolf M&#252;ller

doi:10.3791/61703

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Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

JoVE Journal
生物工学

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JoVE Journal 生物工学

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

8,095 Views

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07:28 min

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October 29, 2020

DOI:

10.3791/61703-v

DOI:

10.3791/61703-v

•

07:28 min

October 29, 2020

•

2 Views

Katarina Cirnski*^1,2, Janetta Coetzee*^1,2, Jennifer Herrmann^1,2, Rolf Müller^1,2

¹Helmholtz Institute for Pharmaceutical Research Saarland, Department of Microbial Natural Products, Helmholtz Centre for Infection Research and Department of Pharmacy,Saarland University, ²Partner Site Hannover-Braunschweig,German Centre for Infection Research (DZIF)

概要

The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.

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Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

概要

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