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Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

8,106 Views

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07:28 min

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October 29, 2020

DOI:

10.3791/61703-v

DOI:
10.3791/61703-v

•

07:28 min
October 29, 2020

•

2 Views
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1Helmholtz Institute for Pharmaceutical Research Saarland, Department of Microbial Natural Products, Helmholtz Centre for Infection Research and Department of Pharmacy,Saarland University, 2Partner Site Hannover-Braunschweig,German Centre for Infection Research (DZIF)

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The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.

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Cirnski, K., Coetzee, J., Herrmann, J., Müller, R. Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry. J. Vis. Exp. (164), e61703, doi:10.3791/61703 (2020).

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