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Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
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JoVE Journal 神経科学
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

6,039 Views

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08:04 min

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November 19, 2020

DOI:

10.3791/61629-v

DOI:
10.3791/61629-v

•

08:04 min
November 19, 2020

•

4 Views
, ,
1Center for Genomic Medicine,Massachusetts General Hospital, 2精神医学科,Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, 4精神医学科,Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program,McLean Hospital

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This protocol details an adapted method to derive, expand, and cryopreserve brain microvascular endothelial cells obtained by differentiating human induced pluripotent stem cells, and to study blood brain barrier properties in an ex vivo model.

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Pong, S., Lizano, P., Karmacharya, R. Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (165), e61629, doi:10.3791/61629 (2020).

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