/
/
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
6,043 Views
•
08:04 min
•
November 19, 2020
DOI:
1Center for Genomic Medicine,Massachusetts General Hospital, 2Department of Psychiatry,Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, 4Department of Psychiatry,Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program,McLean Hospital
Summary
Automatically generated
This protocol details an adapted method to derive, expand, and cryopreserve brain microvascular endothelial cells obtained by differentiating human induced pluripotent stem cells, and to study blood brain barrier properties in an ex vivo model.
Read Article
Cite this Article
Pong, S., Lizano, P., Karmacharya, R. Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (165), e61629, doi:10.3791/61629 (2020).
Copy