ジャーナル

/

/

通过 EFA 去卷和散射分析 SEC-SAXS 数据

JoVE Journal
生化学

This content is Free Access.

JoVE Journal 生化学

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Please note that all translations are automatically generated. Click here for the English version.

通过 EFA 去卷和散射分析 SEC-SAXS 数据

8,302 Views

•

10:59 min

•

January 28, 2021

DOI:

10.3791/61578-v

DOI:

10.3791/61578-v

•

10:59 min

January 28, 2021

•

12 Views

Mark D Tully*¹, Nicolas Tarbouriech², Robert P Rambo³, Stephanie Hutin*⁴

¹European Synchrotron Radiation Facility Structural Biology Group,Structural Biology Group, ²Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, ³Diamond Light Source, ⁴Laboratoire de Physiologie Cellulaire and Végétale,Université Grenoble Alpes/CNRS/CEA/INRA/BIG

概要

SEC-BioSAXS 生物大分子的测量是确定大分子及其复合物溶液结构的标准方法。在这里，我们分析来自两种常见 SEC 跟踪的 SEC-BioSAXS 数据 - 具有完全解析和部分解析峰值的色谱图。我们使用散射和 BioXTAS RAW 演示分析与去卷。

Explore More Videos

SEC-SAXS EFA Deconvolution Scatter Vaccinia Virus DNA Polymerase Background Subtraction Size Exclusion Chromatography Monodispersity Macromolecule Macromolecular Complex Deconvolution Data Analysis

Read Article