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Analysis of SEC-SAXS data via EFA deconvolution and Scatter
JoVE Journal
生化学
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JoVE Journal 生化学
Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

DOI:

10:59 min

January 28, 2021

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  • 00:05Introduction
  • 00:49Primary Data Analysis
  • 02:20Data Deconvolution
  • 04:56Small Angle X-Ray Scattering (SAXS) Property Assessment
  • 08:22Results: Representative EFA Deconvolution and Scatter
  • 10:05Conclusion

概要

自動翻訳

SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.

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