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Analysis of SEC-SAXS data via EFA deconvolution and Scatter
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Biochemie
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JoVE Journal Biochemie
Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

8,318 Views

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10:59 min

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January 28, 2021

DOI:

10.3791/61578-v

DOI:
10.3791/61578-v

•

10:59 min
January 28, 2021

•

12 Views
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1European Synchrotron Radiation Facility Structural Biology Group,Structural Biology Group, 2Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 3Diamond Light Source, 4Laboratoire de Physiologie Cellulaire and Végétale,Université Grenoble Alpes/CNRS/CEA/INRA/BIG

Summary

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SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.

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Tully, M. D., Tarbouriech, N., Rambo, R. P., Hutin, S. Analysis of SEC-SAXS data via EFA deconvolution and Scatter. J. Vis. Exp. (167), e61578, doi:10.3791/61578 (2021).

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