00:47Construction of Bilateral Cannulas, Obturators, and Caps
02:49Construction of Microinjectors
04:10Animal Maintenance and Vaginal Smears
05:31Microinjection of TTX or Vehicle Solutions into Freely Moving Rats
07:27Tissue Processing
08:44Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)