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Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
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神经科学
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JoVE 杂志 神经科学
Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections

DOI:
10.3791/61493-v

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11:04 min

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September 03, 2020

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, , , , , ,
1Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza,Universidad Nacional Autónoma de México, 2Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza,Universidad Nacional Autónoma de México, 3Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza,Universidad Nacional Autónoma de México, 4Facultad de Ciencias,Universidad Nacional Autónoma de México, 5Department of Basic Research, Instituto Nacional de Geriatria,Ministry of Health

Chapters

  • 00:04Introduction
  • 00:47Construction of Bilateral Cannulas, Obturators, and Caps
  • 02:49Construction of Microinjectors
  • 04:10Animal Maintenance and Vaginal Smears
  • 05:31Microinjection of TTX or Vehicle Solutions into Freely Moving Rats
  • 07:27Tissue Processing
  • 08:44Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)
  • 10:08Conclusion

Summary

自动翻译

自动翻译

This protocol describes the construction of a low-cost microinjection system, its stereotaxic implantation into deep-brain structures and the procedure for timed microinjections of tetrodotoxin in awake and unrestrained rats. The goal is to reveal the participation of hypothalamic structures in the regulation of ovulation by inhibiting their neural activity.

Tags

Keywords: Rat BrainOvulation RegulationReversible InactivationTetrodotoxinMicroinjectionsEstrous CycleNeurobiologyPhysiological Processes

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