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Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
章
- 00:04Introduction
- 00:34Preparation of Agarose Pads
- 01:28Equilibrating Materials to Experimental Temperature
- 02:06Inoculation of Agarose Pads
- 03:13Microscope Setup for Live Imaging
- 05:32Results: P. aeruginosa Behavior when Cocultured with S. aureus
- 07:47Conclusion
This live-bacterial cell imaging protocol allows for visualization of interactions between multiple bacterial species at the single-cell level over time. Time-lapse imaging allows for observation of each bacterial species in monoculture or coculture to interrogate interspecies interactions in multispecies bacterial communities, including individual cell motility and viability.
