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Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

English

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6,511 Views

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08:33 min

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August 05, 2020

DOI:

10.3791/61376-v

DOI:
10.3791/61376-v

•

08:33 min
August 05, 2020

•

6 Views
, ,
1Department of Microbiology and Immunology, Carver College of Medicine,University of Iowa

Summary

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This live-bacterial cell imaging protocol allows for visualization of interactions between multiple bacterial species at the single-cell level over time. Time-lapse imaging allows for observation of each bacterial species in monoculture or coculture to interrogate interspecies interactions in multispecies bacterial communities, including individual cell motility and viability.

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Yarrington, K. D., Sánchez Peña, A., Limoli, D. H. Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions. J. Vis. Exp. (162), e61376, doi:10.3791/61376 (2020).

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