This protocol serves as a comprehensive tutorial for standardized and reproducible mixing of viscous materials with a novel open source automation technology. Detailed instructions are provided on the operation of a newly developed open source workstation, the usage of an open source protocol designer, and the validation and verification to identify reproducible mixtures.
Eggert, S., Kahl, M., Kent, R., Gaats, L., Bock, N., Meinert, C., Hutmacher, D. W. An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion. J. Vis. Exp. (181), e61261, doi:10.3791/61261 (2022).